منابع مشابه
Far-field autofluorescence nanoscopy.
We demonstrate far-field optical imaging at the nanoscale with unlabeled samples. Subdiffraction resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning. Our approach is based on the insight that nanoscopy methods...
متن کاملFar - Field Optical Nanoscopy
37. E. Evans, Annu. Rev. Biophys. Biomol. Struct. 30, 105 (2001). 38. G. I. Bell, Science 200, 618 (1978). 39. For adhesive interactions important in soft tissues and organs of eukaryotic cell systems, the average times toff° reported for spontaneous dissociation of ligand/receptor bonds range from a fraction of a second to 100 s or more. 40. Because of thermal activation, the appropriate unit ...
متن کاملFar - Field Optical Nanoscopy Stefan
37. E. Evans, Annu. Rev. Biophys. Biomol. Struct. 30, 105 (2001). 38. G. I. Bell, Science 200, 618 (1978). 39. For adhesive interactions important in soft tissues and organs of eukaryotic cell systems, the average times toff° reported for spontaneous dissociation of ligand/receptor bonds range from a fraction of a second to 100 s or more. 40. Because of thermal activation, the appropriate unit ...
متن کاملTwo-color far-field fluorescence nanoscopy.
We demonstrate two-color fluorescence microscopy with nanoscale spatial resolution by applying stimulated emission depletion on fluorophores differing in their absorption and emission spectra. Green- and red-emitting fluorophores are selectively excited and quenched using dedicated beam pairs. The stimulated emission depletion beams deliver a lateral resolution of <30 nm and 65 nm for the green...
متن کاملPlasmonics meets far-field optical nanoscopy.
Plasmonics and near-field optical nanoscopy both deal with expanding optics into the subwavelength regime. However, these two fields have so far followed parallel paths of development and only recently have researchers started to explore combinations of their concepts with potential synergy. In this Perspective, we provide an up-to-date summary of the successful combinations reported and give i...
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ژورنال
عنوان ژورنال: Nano Letters
سال: 2010
ISSN: 1530-6984,1530-6992
DOI: 10.1021/nl1027638